466 Cancer-associated fibroblast activation predicts progression, metastasis and prognosis of cutaneous squamous cell carcinoma

Cutaneous squamous cell carcinoma (cSCC) possesses metastatic potential and in case the prognosis is poor. At present, there are no established biomarkers for assessment of metastasis risk or primary cSCC. Cancer-associated fibroblasts (CAFs) promote progression cancer, but limited evidence their role Here, we have examined CAF markers We used multiplexed fluorescence immunohistochemistry to profile landscape non-metastatic human cSCCs, actual metastases, premalignant benign epidermal lesions. Quantitative high-resolution image analysis was performed with two separate panels antibodies results were correlated clinical histopathological parameters including disease-specific mortality. Increased stromal expression fibroblast activation protein (FAP), α-smooth muscle actin (α-SMA), secreted acidic rich cysteine (SPARC) associated ultraviolet-induced lesions invasive Elevation FAP platelet-derived growth factor receptor-β (PDGFRβ) cSCC stage. High PDGFRβ periostin poor prognosis. PDGFRα-/PDGFRβ+/FAP+ subset invasion independently predicted cSCC-specific survival. Fibroblast evolves during alone phenotype appear as stage,